Focusing on the overlapping zone, we obtained an optimal cutting point of GAPDH-CD45 = 2 (40-Ct Mean) and VIL1-CD45 = −7.5 (40-Ct Mean), rendering 72.7% of true positive metastatic CRC patients with a 9.09% of false positives (Fig. 3B). Here, VIL1 is linked to colorectal carcinoma.