CXCL8 and renal cell carcinoma: To determine if Nox-dependent ROS mediate hypoxia-induced IL-6 and IL-8 production, we pre-incubated RCC 786-O cells with buffer alone (-), the Nox inhibitor, diphenyleneiodonium (DPI) or the ROS scavenger, N-Acetyl Cysteine (NAC) for 30 min and subjected to hypoxia or normoxia for 48 hours.