Since 24 h of UO leads to HIF-2α stabilization without the induction of HIF-1α, UO can be widely employed in the research work concerning the role of HIF-2α in various renal diseases besides ischemic acute renal failure, for example, nephrotoxic acute kidney injury, radiocontrast nephropathy, and acute glomerulonephritis. This evidence concerns the gene EPAS1 and acute kidney injury.