Based on the role of FSCN1 in promoting breast cancer progression [22,33,34] and a target gene of miR-133a in esophageal squamous cell cancer and bladder cancer [35,36] as well as identification of one potential binding sites of miR-133a in the FSCN1 3'-UTR (Figure 3A), we first transfected miR-133a mimic into MCF-7 cells and assay FSCN1 mRNA levels in these cells. The gene discussed is FSCN1; the disease is urinary bladder carcinoma.