To determine the subcellular localization of zebrafish Alr, plasmids expressing Alr-V5 and Alr-EGFP fusion proteins were generated and transiently expressed in HepG2 (human hepatocellular carcinoma cell), HEK293T (human embryonic kidney cell) as well as ZFL cells (zebrafish liver cell). The gene discussed is GFER; the disease is hepatocellular carcinoma.