To further confirm and characterize the interaction between Mieap and NIX/BNIP3, exogenous Mieap α, Mieap β, FLAG-NIX, and FLAG-BNIP3 proteins were expressed through infection using Ad-Mieap α or Ad-Mieap β and transfection with plasmids expressing FLAG-NIX or FLAG-BNIP3. This evidence concerns the gene BNIP3 and infection.