CASP3 and infection: Although NIX and BNIP3 were actually overexpressed in HCT116 cells by infection with the high dose of Ad-BNIP3 and Ad-NIX (Figure S4), we observed neither of the MMP reduction (Figure S5A), the increased dead cells (Figures S5B and S6), nor the caspase-3 activation (Figure S5C) at both early (48 h after infection) and late phases (98 h after infection).