Replacing the culture media at 14 days after infection with Ca2+-free buffer showed Ca2+-independent increase in GABA release and was seen in HIV1-CMV-GAD65-GFP (baseline 4±3nmol→445±95 nmol at 3 hrs; p<0.01) but not in HIV1-CMV-GFP-infected cells (Fig. 2J). This evidence concerns the gene GAD2 and infection.