To assess whether the sumoylation of NFRKB might account for the apparent higher molecular weight, we performed immunoprecipitation from PBMC protein lysates of two MCNS relapse samples and eluates were detected either with anti-sumo-1 or anti-sumo-2/3 antibodies and then reprobed with anti-NFRKB. The gene discussed is SUMO2; the disease is lipoid nephrosis.