To investigate whether Sp1 could be the target of IFI16, causing the modulation of IR-1 activity in the context of HCMV infection, nuclear extracts from AdV IFI16- or AdV LacZ-transduced HELFs infected at an MOI of 200 for 24 h and then with HCMV at an MOI of 2 for 24 h were analyzed by EMSA using an IR-1 oligonucleotide probe. Here, NISCH is linked to cytomegalovirus infection.