Our spheroid cells also gained expression of human ACTC1 (αSMA) and VIM (Vimentin) mesenchymal markers, similar to ovarian cancer cell-formed spheroids [43], and doxorubicin-selected MCF-7/ADR cells [44] indicating that an EMT had occurred during cancer progression of the CICs [45]. Here, ACTA1 is linked to ovarian cancer.