Our spheroid cells also gained expression of human ACTC1 (αSMA) and VIM (Vimentin) mesenchymal markers, similar to ovarian cancer cell-formed spheroids [43], and doxorubicin-selected MCF-7/ADR cells [44] indicating that an EMT had occurred during cancer progression of the CICs [45]. The gene discussed is ACTC1; the disease is ovarian cancer.