BMP2 and persistent truncus arteriosus: As shown in Figure 5A, inhibition ROCK activity with Y-27632 effectively suppressed pressure overload-induced collagen deposition in TAC mice at 2 weeks after TAC; however, exogenous supply of BMP-2 could also reduce the formation of collagens as well as decreasing collagen type I and type III gene expression (Fig. 5A and B).