In order to compare the functional response of the indentified polymorphisms, the TLR5 sequence of the IBD risk-protective haplotype (G22A haplotype GTT) and the IBD risk-associated haplotype (G22A haplotype ACC) [16] were cloned in to pcDNA3.1 plasmids containing YFP to monitor successful expression. The gene discussed is TLR5; the disease is inflammatory bowel disease.