This variability may be the result of the different methods used to assess TP53 mutations (SSCP, denaturing gradient gel electrophoresis, temperature gradient gel electrophoresis and direct sequencing), the type of tumour storage (fresh/frozen tissue and paraffin embedded blocks), an intrinsic tumoural heterogeneity, and, in addition, more specific features of the patient cohorts in the study, in particular, histopathological staging and grading of the tumour. Here, TP53 is linked to neoplasm.