Tumor tissue lysates of two bevacizumab-sensitive models, GXF97 (high VEGF expression) and 4-1ST (VEGF level equivalent to SC-10-JCK), and one bevacizumab-insensitive model, SC-10-JCK, with or without bevacizumab, were added to HUVEC, and the phosphorylation level of VEGFR2 in HUVEC was tested by western blot (Figure 3A). The gene discussed is VEGFA; the disease is neoplasm.