The invasive phenotype of RWPE-1 cells was induced by EZH2 overexpression as observed above (Figure 1D), but it was significantly suppressed by cooverexpression of TIMP3 (Figure 8B), providing direct evidence that the repression of TIMP3 by EZH2 results in increased invasive activity of prostate cancer cells. Here, TIMP3 is linked to Familial prostate cancer.