TIMP3 and Familial prostate cancer: To assess the functional link between the downregulation of TIMP3 by EZH2 (Figure 3) and the decreased invasive phenotype of prostate cancer cells by EZH2 knockdown (Figures 1C and 1D), we overexpressed EZH2 and TIMP3 separately or together in RWPE-1 cells (Figure 8A) and examined the invasive activity of the cells using Transwell Boyden chamber assay (Figure 8B).