Although we can not determine the exact reasons for the discrepancies between Cho's study and ours, the phenotype of obesity in RGS5 KO mice are evident in our experiment and KO mice exhibited an increased body weight through increasing calorie intake, promoting adipocyte differentiation and hypertrophy in the adipose tissue and accelerating ectopic fat deposition in the liver. The gene discussed is RGS5; the disease is obesity due to melanocortin 4 receptor deficiency.