We recently showed that the CD33-CAR approach effectively enhances the antileukemic activity of cytokine induced killer (CIK) cells [18], and, with this study, we extend our observation to EBV-CTLs, whose efficacy in the clinical setting has been widely documented, demonstrating that CD33-specific CAR-expressing EBV-CTLs can be redirected towards human CD33+ AML blasts in vitro and in vivo in a xenograft NOD-SCID mice model of AML. This evidence concerns the gene CD33 and acute myeloid leukemia.