We found that target genes such as CDH1 (miR-1/206), ATM (miR-18a/b), KLF6 (miR-18a/b and miR-181c), Smad2(miR-18a/b, miR-1/206 and miR-149), Dicer(miR-18a/b) were down expressed with the development of NPC, while BCL2L2 (miR-29a/b/c and miR-203), E2F3(miR-34b/c and miR-429), ETS2(miR-429), MYB(miR-429) and YY1 (miR-29a/b/c and miR-34b/c) were overexpressed during the development of NPC. The gene discussed is SMAD2; the disease is nasopharyngeal carcinoma.