To investigate the effect of hyperglycemia on SUR-2A and Kir6.2 expression, we treated neonatal rat cardiac myocytes with high concentrations of glucose (30 mmol/l; HG) in medium with and without 100 nmol of tiron, a superoxide anion scavenger[20] and after incubation we examined the effects on SUR-2A and Kir6.2 expression using western blot analysis (Figure 5). This evidence concerns the gene KCNJ11 and Hyperglycemia.