To model tauopathies therefore, most studies have utilised the UAS-GAL4 expression system to target the expression of either wt or mutant human (and even bovine, rodent, and Drosophila) tau to specific neuronal or glial cells in both larvae and adult Drosophila. In many of these studies, the human tau expressed is highly phosphorylated, with some studies reporting that it also becomes misfolded and/or insoluble [33, 34, 53]. This evidence concerns the gene MAPT and tauopathy.