In this study concentrations of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) were analyzed from undiluted, cut vitreous samples from patients with age related macular degeneration (ARMD), diabetic macular edema (DME), and central retinal vein occlusion (CRVO), which qualified for intravitreal injections on one clinical day. The gene discussed is VEGFA; the disease is diabetic macular edema.