Cultured ELISPOT assays clearly confirmed responses against individual minigenes from two pools targeted by T cells from subject ND2, one derived from epithelial adhesion molecule, EpCAM in pool #308, and another encoding a portion of glioma pathogenesis associated protein, GLIPR1 in pool #85 (Fig. 2, panels c and d). This evidence concerns the gene EPCAM and central nervous system cancer.