We applied this approach to RASopathies with the aim of finding molecular correlates of the mutational status in PBMCs, focusing on the two genes most frequently mutated in NS, PTPN11, and SOS1, and on SHOC2, which has been recently discovered to cause NS/LAH, a disorder with clinical overlap with the former [Cordeddu et al., 2009]. Here, SOS1 is linked to Netherton syndrome.