We demonstrate successful cloning of three different cDNA for proteins of different molecular weights, Bax (MW: 26 kDa), catalase (MW: 60 kDa) and p53 (MW: 53 kDa) into the adapted vector, and protein overexpression from the transduced constructs in the LNCaP human prostate cancer cell line. The gene discussed is CAT; the disease is prostate cancer.