ANXA5 and pancreatic neoplasm: In this study, Hoechst 33342 nuclear staining and FACS analysis revealed that treatment of BxPC-3 pancreatic cancer cells with alpinetin for 48 h increased condensed chromatin and Annexin V-positive and PI-negative populations in a dose-dependent manner, which suggested that part of the alpinetin induced suppression was mediated through the induction of apoptosis rather than the necrosis.