Furthermore, expression of WT1 in osteosarcoma cell lines, Saos-2 and U20S can alter signaling pathways and induce apoptosis [20], and plasmid-mediated transfection of WT1-KTS isoforms into in HCC cell lines, Hep3B and HepG2 also induced apoptosis [10]. This evidence concerns the gene WT1 and osteosarcoma.