Few studies have used exon-specific primers to study all variant exon-containing CD44 isoforms in HNSCC, most have used P1/P2 or equivalent primers [9], [29], to detect total CD44 or CD44s. Two studies have used exon-specific primers for CD44 v3, finding that v3 transcripts are higher in tumour tissues than normal [29] and suggested that it is linked with growth and migration [30] in HNSCC. Here, CD44 is linked to head and neck squamous cell carcinoma.