To examine whether the silencing of eIF4E or eIF(iso)4E genes has an impact on the outcome of viral infection, homozygous T2 from RNAi-4E-1, RNAi-4E-10, RNAi-iso4E-1 and RNAi-iso4E-6 lines were challenged with strains of the following 7 potyviruses: PVY, TEV, PepMoV, Ecuadorian rocotto virus (ERV), Pepper severe mosaic virus (PepSMV), Pepper yellow mosaic virus (PepYMV), and Potato virus V (PVV). This evidence concerns the gene EIF4E and viral infectious disease.