To identify the pathway responsible for IL-1β and IL-18 secretion in response to infection with B. pseudomallei, bone marrow-derived macrophages (BMDM) or dendritic cells (BMDC) derived from WT mice or mice deficient in the inflammasome components ASC, NLRP3, NLRC4, or caspase-1 were infected in vitro with B. pseudomallei and secretion of IL-1β in culture supernatants was measured. This evidence concerns the gene NLRC4 and infection.