In order to select the appropriate cell lines for our study, we evaluated the level of CHD5 protein in four colon cancer cell lines by Western blot analysis (Fig. 1C), which included two previously established cell lines (RKO and HCT-116) and two CHD5 transfected cell lines (RKO-S and RKO-AS). The gene discussed is CHD5; the disease is colonic neoplasm.