A pooled analysis of 1041 patients from three clinical trials assessing adjuvant IFN in stage IIB-III melanoma (Nordic Study, EORTC 18952, and EORTC 18991) evaluated ELSA-determined YKL-40 levels in serum samples that were collected at study outset, during treatment and at followup every three to six months for up to 10 years. This evidence concerns the gene CHI3L1 and melanoma.