The decreased vascularization in treated tumors was further validated by immunostaining of corresponding tumor sections and subsequent quantification of CD31-positive area fractions (control group, 4.8 ± 0.50%; therapy group, 1.7 ± 0.37%; **P < 0.001; Figure 4D; representative pictures are shown in Figure 4E). This evidence concerns the gene PECAM1 and neoplasm.