To confirm the identified protein and to validate the results of SELDI-TOF MS analysis, Western blot analyses of Protein S100A10 in whole cell lysates from 8 CRC cell lines (HCT15, COLO-320, LS174T, SW620, SW480, HT29, DLD-1 and HCT116) were performed. This evidence concerns the gene S100A10 and colorectal carcinoma.