IRF3 and infection: Figure 6A and 6B show that fibroblasts undergo nuclear accumulation of IRF3 following infection with DENV-2. While in mock and uninfected cells, IRF3 distribution was predominantly cytoplasmic. By contrast, DENV infection of skin fibroblasts failed to induce IRF7 nuclear translocation at 24 or 48 h post-infection (Figure 6E), the location of the molecule was mainly cytoplasmic. At 72 h, when some changes were detected, the cells were already damaged and highly granular (Figure 6D).