Three approaches were taken: 1) to establish a robust MQDL protocol to detect and quantify a panel of gene expressions on the CRPC LTL-313 model tissue specimen with results compared with SQDL; 2) to use this established quantitative MQDL protocol to confirm if activation of c-Met and EMT occur in the CRPC LTL-313 tissues maintained in the castrated hosts; and 3) to demonstrate by the standardized MQDL protocol the activation of c-Met and EMT induction in both primary and skeletal metastatic human prostate cancer tissues. This evidence concerns the gene MET and Familial prostate cancer.