To determine whether MCPIP1 is involved in LPS preconditioning-induced tolerance to ischemic brain injury, MCPIP1 knock out or wild type mice were treated with a low dose of LPS (0.2 mg/kg), and 24 hours later these mice were subjected with MCAO for 90 min followed by 48 hours reperfusion. The gene discussed is ZC3H12A; the disease is brain injury.