Similarly, He et al. reported that loss of WIF-1 expression was associated with promoter hypermethylation in colorectal cancer, and that restoration of WIF-1 function, Wnt-1 siRNA, or a monoclonal anti-Wnt-1 antibody induces significant apoptosis in colorectal cancer cells which contain downstream mutations and expressing Wnt-1 [46]. This evidence concerns the gene WNT1 and colorectal cancer.