In the paper by Tagawa et al the authors found defective expression of dysferlin by IH, 19% of patients with LGMD and 50% of patients with other neuromuscular diseases, and established four different patterns of dysferlin staining (normal, negative, faint and abnormal cytoplasmic accumulation) The authors concluded that other proteins are necessary for a normal localization of dysferlin at the sarcolemma. Here, DYSF is linked to limb-girdle muscular dystrophy.