TIMP1 and peritonitis: E. faecalis V583 mutants that mapped in the epa region (ef2196 and ef2198) or adjacently (ef2167 and ef2170) all displayed multiple phenotypes In OG1RF, mutants in epaB (ef2197) and epaE (ef2194) were attenuated for virulence in a mouse peritonitis model [64], more susceptible to PMN-killing [53], and were deficient in translocation across enterocyte-like T84 cells [65] and biofilm formation [54], [66].