We had developed and characterized an inducible dry eye model where mice subjected to cholinergic blockade and chronically exposed to a drafty environment develop disruption of corneal barrier function, increased production of pro-inflammatory cytokines and metalloproteinases (MMP), activation of mitogen-activated protein kinase (MAPK) intracellular pathways and production of cornified envelope protein precursors [27], [37]–[39] mimicking several features of human dry eye patients [40]–[42]. This evidence concerns the gene WNK2 and Keratoconjunctivitis sicca.