In order to study the antiviral response against these viruses in VEFs, cells were infected with CPXV, LV, PUUV-Kazan-E6 and TBEV, and levels of mRNA expression for IFN-β and Mx2 were analyzed with Q-PCR at 6, 12, 24, 48 and 96 hours after infection. This evidence concerns the gene IFNB1 and infection.