To further test our hypothesis that reduced levels of individual Rps in the PG of Minute mutants might restore proliferation in the cycEJP eye by inducing a developmental delay, we sought to reduce Rp expression in the PG using AmnC651-Gal4 which drives expression in the PG [41] and UAS-Rp RNAi for RpS6, RpS13 or RpL38. We first demonstrated the RNAi was able to reduce RpS6 protein by knocking down specifically in the PG, and staining with an anti-RpS6 antibody (Figure S3A). This evidence concerns the gene RPS6 and Global developmental delay.