EGFR and neoplasm: Although sequencing analysis cannot be considered 100% accurate, owing to the low sensitivity of direct sequencing [30,31], the high tumour cellularity after macrodissection, as well as the quality of the DNA isolated from our frozen TNBC samples, allow us to be confident in our results and to conclude that our TNBC samples did not present EGFR-activating mutations that are detectable using the routine techniques used in molecular biology laboratories.