After 3 days culture in SCM, NSE was downregulated in 2 of the 5 GBM cell lines (fold change in LN229, 0.5 ± 0.05, p < 0.001; U87, 0.5 ± 0.04, p < 0.001) and slightly upregulated in GaMG (1.4 ± 0.17, p = 0.041, figure 2A). This evidence concerns the gene ENO2 and glioblastoma.