ENO2 and glioblastoma: NSE was significantly upregulated in all the GBM cell lines for both short-term (fold change in GaMG, 2.3 ± 0.32, p = 0.016; LN229, 1.3 ± 0.01, p < 0.001; U251, 3.2 ± 0.02, p < 0.001; U87, 1.7 ± 0.15, p = 0.009) and long-term culture (fold change in A172, 4.3 ± 1.32, p = 0.046; GaMG, 1.8 ± 0.08, p < 0.001; LN229, 1.9 ± 0.12, p < 0.001; U251, 2.5 ± 0.45, p = 0.014; U87, 1.9 ± 0.32, p = 0.032) except A172 cells cultured for 4 days.