The analysis of RT-PCR products showed that TLR4 and MD-2 were constitutively expressed in 4 out of 8 human colorectal carcinoma cell lines, including Colo 205, RKO, SW480 and SW620, whereas TLR4 and MD-2 were present respectively in only two cell lines such as DLD-1 and HCT-29 (Fig. 1A). This evidence concerns the gene TLR4 and colorectal carcinoma.