To determine whether IRGM is involved in autophagosome formation observed upon MeV, HCV and influenza A infections we have abrogated IRGM expression using specific si-RNA (Figure S6A–G), prior to infection in GFP-LC3-HeLa cells, GFP-LC3-Huh 7.5 or GFP-LC3-A549 cells, respectively. This evidence concerns the gene MAP1LC3A and infection.