MSLN and cancer: Figure 7 shows that, as expected, the anti-mesothelin ELISA assay detected soluble mesothelin in all ascites fluids as well as in conditioned media from ovarian cancer cell lines and from 293 MESOIg (a transformed cell line that expresses a GPI anchor-truncated mesothelin fused to an Ig domain [57]) (Fig. 7A). Endo-A meso ELISA also detected soluble mesothelin in ascites fluids and conditioned media from cancer cell lines. However, Endo-A meso ELISA could not detect soluble mesothelin released by 293 MESOIg cell line that is truncated for the GPI anchor (Fig. 7B).