Furthermore, the IRGM1 expression was knocked down by siRNA to further investigate its role in IFN-γ/Con A-induced LMP-associated necrosis (Figure 4C).We found that the IFN-γ/Con A-caused increase of LMP in hepatoma cells was significantly inhibited by IRGM1 siRNA, suggesting that IRGM1 is involved in IFN-γ/Con A-caused LMP change (Figure 4D). The gene discussed is IRGM; the disease is hepatocellular carcinoma.