Treatment with both 10 μM and 100 μM SIRT1 significantly decreased the number of NP cells dose-dependently in overall samples, compared with the control and negative control groups (total: 10 μM, -34.4% and 100 μM, -40.4% (Figure 5a); LDH and LSS: 10 μM, -21.5% and 100 μM, -34.4% (Figure 5b); and idiopathic scoliosis: 10 μM, -32.3% and 100 μM, -41.5% (Figure 5c); P < 0.05). This evidence concerns the gene SIRT1 and idiopathic scoliosis.