Results showed a significant difference in the number of CA repeats between the case and the control groups (p=0.01).Conclusion: This study confirms the crucial role of TGF-β3 in the fusion of palatal shelves during development and further, provides novel evidence of TGF-β3 gene polymorphism in the etiology of nonsyndromic cleft lip and palate in Indian subpopulation. Here, TGFB3 is linked to cleft lip.